Application of the Methylated Markers (Spectrin Beta and DEAD-Box Protein) for Definitive Differentiation Between Fresh and Aged Semen by evaluating Their Role in Identifying Semen From Mixed Body Fluids

Background: Semen identification is assumed a crucial proof of sexual assault. Moreover, body fluids at the crime scene human being, such as blood, semen, and saliva, are often mixed. Methods: Hence, in our study, we aimed to use methylation analysis targeting DNA epigenetic markers Spectrin beta chain (B_SPTB_03) DEAD-box protein (DDX4) differentiate between fresh semen (less than 4 hours) aged (after 24 well alone mixed with other (blood saliva) dried state. Results: Our findings showed statistically significant differences patterns SPTB DDX4 loci distinguish from old samples. We were able obtain two novel cutoff values which (52.25) marker (70.75) marker. Conclusion: It concluded that approach based on (B_SPTB_03 DDX4) successfully identified semen-derived alleles stains, hence it recommended be employed forensic practice.